Kutsvaga Kwakaitika uye Kukosha Kwazvo
Mukugadzirwa kwemishonga yekudzivirira chirwere cheFMD, zvinhu zvinodzivirira masero zvine huwandu hwakawanda hwezvinhu zvakasara mumasero. Kana zvikaiswa zvakananga mu 0.2 μm sterilizing filtration, ma filter membranes anogona kukurumidza kusvibiswa, zvichideredza kushanda zvakanaka uye zvinogona kukanganisa kupinda kwemvura uye goho re target antigen (146S viral particles).
Chidzidzo ichi chinotarisa pamibvunzo mitatu inokosha:
Kuenzanisa mashandiro ekusefa pamusoro uye kusefa kudzika maererano nekubuda kwemvura, kuderedza kusviba, uye kudzivirira mafirita ekuuraya utachiona ari pasi.
Kusimbisa mukana wekuti mafirita akasimba ane chaji yakanaka (strong positive charged depth filters) akwanise kubviswa mumaseru.
Kugadzira zvirongwa zvekusefa zvinokwanisa kukura zvakagadzirwa zvichienderana nemvura yekurima ine mazinga akasiyana e turbidity, kuti ienderane nezvinodiwa zvekugadzirwa kwemaindasitiri.
Kuedza Kwepfungwa Nemhedzisiro
Kusefa Kwepamusoro vs. Kusefa Kwekudzika
Kuedza:Mvura yekudzivirira yeFMD isina kushanda yakaseferwa uchishandisa 0.45 μm polypropylene surface membrane uye mafirita ekudzika kwemvura, zvichiteverwa ne 0.2 μm sterilizing filtration.
Zvakawanikwa:
Kutonhora kwekutanga: 77 NTU.
Kusefa kudzika:simba remagetsi rinosvika 641 L/m²; kutonhora kwakaderera kusvika 9.65 NTU (kwakajeka uye kwakajeka).
Kusefa pamusoro:simba remagetsi raisvika 46 L/m² chete; kusviba kwakaramba kuri 41 NTU.
Kuchenesa tsvina kunodzika pasi:Mushure mekusefa zvakadzika, mafirita e 0.2 μm aive ne throughput ye 655 L/m², zvichienzaniswa ne 91 L/m² chete mushure mekusefa pamusoro - kuvandudzwa kwe 7.2 ×.
146S goho:Kusefa kwakadzika kwakakwira kupfuura kusefa pamusoro.
Mhedziso:Kudzika kwekusefa kwaive ne13.9× yakakwira kupfuura kusefa kwepamusoro, kusviba kwetsvina kwaive kwakaderera ne76.5%, uye hupenyu hwesefa hwekuuraya utachiona hwakawedzerwa kupfuura 7×. Kubva pamaonero emutengo negoho, kusefa kwakadzama kwakanakisa.
Kushaikwa Kwemvura Yakanaka vs. Kudzika Kwemvura Yakasimba Kwakashaikwa
Kuedza:Mhando mbiri dzemapepa ekusefa ane saizi yakafanana yepore asi huwandu hwakasiyana hwekuchaja (hwakajairika uye hwakasimba) dzakaedzwa pamvura dzeFMD dzisina kushanda.
Zvakawanikwa:
Mapurogiramu ekushandisa simba uye kumanikidzwa zvaive zvakafanana.
Kudzika kwakanaka kwakajairikasefa:tsvina yekusvina 25 NTU.
Kudzika kwakasimba kwakanakasefa:filtrate turbidity 9.65 NTU (38.6% chete yezvakajairika).
Kukwanisa kuchaja kwakasimba kunobva kuma resin ligands akakosha anowedzera electrostatic adsorption.
Mishumo yezvinyorwa inosimbisa kuti mafirita akasimba ehupamhi hwepfungwa anogonawo kubvisa mapuroteni emasero emubatsiri (HCP) neDNA.
Mhedziso:Mafirita akasimba ehupamhi hwemvura anopa kuderedza kusviba kwemvura zviri nani (<10 NTU), anodzivirira kusvina kwemvura zvakanyanya/kuuraya utachiona, uye anobvisa tsvina yakawanda ine chekuita nemaitiro.
1. Nheyo dzeUnyanzvi uye Mabhenefiti eKusefa Kwakadzika
Kusefa pamusoro:Inobata zvidimbu zviri pamusoro peganda chete; kunyanya kuvharwa kwakavakirwa pahukuru.
Kusefa kudzika:Inobvisa zvidimbu mukati me matrix kuburikidza ne entrapment uye electrostatic adsorption.
Zvakanakira zvikuru:
Hapana asbestos (kusiyana nemidziyo yekusefa yekutanga).
Yakagadzirwa nefibers dze cellulose dzakachena zvakanyanya, zvinhu zvinobatsira pakusefa, uye polymeric resins.
Mapoka anoshanda ane chaji yakanaka anopa kunyungudika kwakasimba kwezvidimbu zvidiki netsvina.
Inotevedzera zvizere zvinodiwa nemutemo we biopharmaceutical.
2. Maitiro Ekusefa Anogona Kuwedzerwa eMatanho Akasiyana eKushanduka Kwemhepo
Zvichibva paongororo dzekuenzanisa:
Zvakadererakusvibazvinwiwa:kupfapfaidza kwakareruka kwakakwana.
Pakati nepakatikusvibazvinwiwa:Kusefa kudzika kwemadziro maviri kunokurudzirwa.
Yakakwirirakusvibazvinwiwa:Kusefa kwakasimba kwehudzamu hwemuviri kunodiwa kuderedza kusviba kuri pasi pe10 NTU, zvichiita kuti pave nekugadziriswa kwakanaka kwemvura iri pasi uye kubereka maantigen akawanda.
3. Kushandiswa Kwechigadzirwa: Mafirita eGreat Wall Filtration Depth
Great Wall Filtration inopa masisitimu ekusefa akagadzirirwa maitiro emishonga yekurapa.
Zvinhu zvikuru:
Mapepa efirita ane hurefu hwakakura ekubvisa masero, marara, uye zvinhu zvikuru zvakasimba.
Inoshanda kune mhuka dzinoyamwisa, bacteria/mbiriso lysates.
Inokwanisa kubvisa HCP, DNA, mavhairasi, endotoxins, uye mishonga inorwisa furo.
Dhizaini diki, inogona kukura yakatwasuka, yakakodzera kushandiswa mumutsara kana padanho rakafanana.
Mwero wekufamba kwemvura wakadzikama, hupenyu hwebasa hurefu, uye mutengo wakaderera wekushanda.
Mhedziso
Tekinoroji yakasimba yekusefa yakachajwa zvakanaka inovandudza kushanda zvakanaka kwekuongorora uye kudzoreredza maantigen mukugadzirwa kwemishonga yeFMD. Kunze kwemishonga yeFMD, inopa nzira yakavimbika yekuchenesa mune zvimwe zvipenyu. Sezvo kugadzirisa maitiro ebioprocess kuchienderera mberi, tekinoroji yepamusoro yekusefa icharamba yakakosha pakugadzira mishonga yekudzivirira yakachengeteka uye inoshanda.
Chinyorwa ichi chakavakirwa padata rechokwadi rekuyedza chete.Kusimbiswapasi pemamiriro chaiwo ekugadzira zvinokurudzirwa.
